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KMID : 0861020130280040017
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Korea Journal of Herbology
2013 Volume.28 No. 4 p.17 ~ p.23
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Comparison of geniposide quantification and antioxidant effect among the various parts of Gardeniae fructus
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김승택:Kim Seung-Taik
이장천:Lee Jang-Cheon/이부균:Lee Boo-Kyun/이금산:Lee Guem-San
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Abstract
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Objectives : The objective of the paper was to study cutting method of Gardeniae frutus, that tends to show symptom of separations in a packing, in accordance with the comparison experiment of quantification and antioxidant effect proceed with the intact Gardeniae fructus(Gf), seed of Gardeniae fructus (Gs) and the pericarp of Gardeniae fructus (Gp) separately.
Methods : The Gf, Gs, and Gp were extracted using 80% MeOH, followed by quantizing geniposide contained in each group. A MTT assay was conducted and ROS generation and NO production were measured for comparing its antioxidant effect.
Results : As a result of quantizing geniposide contained in the Gf, Gp, and Gs, respectively, the geniposide content was shown to be the highest in the Gs. MTT assay showed that no cytotoxicity was observed in the groups treated with Gf, Gp, and Gs, respectively, at a dose of 500 μg/ml. The ROS generation was shown to have more significantly decreased in the group pretreated with Gf 500 μg/ml than in the group treated with LPS. The NO level was shown to have more significantly decreased in the group pretreated with Gp 500 μg/ml than in the group treated with LPS.
Conclusion : As the geniposide content and antioxidant effect of Gf varies according to its each part, it is recommended that Gf should be distributed as an intact form other than segregation in packing.
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KEYWORD
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Gardeniae fructus, seed of Gardeniae fructus (Gs), pericarp of Gardeniae fructus (Gp), antioxidant effect, ROS, NO
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